Regulatory

Part:BBa_K110004

Designed by: James DiCarlo   Group: iGEM08_Johns_Hopkins   (2008-05-30)


Alpha-Cell Promoter Ste3

This promoter was isolated by taking 500bp upstream of the STE3 gene of S. cerevisiae. This promoter will be activated when alpha factor is expressed in an alpha haploid yeast cell.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 107
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 107
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 107
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 107
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 107
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//rnap/eukaryote/yeast
//direction/forward
//chassis/eukaryote/yeast
//promoter
//regulation/positive
Parameters
negative_regulators
positive_regulators1